ISO 846:2019 pdf free

ISO 846:2019 pdf free.Plastics一Evaluation of the action of microorganisms
If the test specimens contain biocides and the test is intended to show the biocidal efficacy of an active substance intended to protect the material from biodeterioration, then for test series I two sets of test specimens have to be tested: one without a biocide (sub-test series a) and one test series with the biocide (sub-test series b). Therefore, 10 test specimens in total need to be inoculated. ldeally, these test specimens should be identical except for their biocide content. This test set-up would allow the demonstration of the basic preservative efficacy for an active substance.
These control specimens are comprised of non-corrodible stainless steel, which contains no nutrient source for fungi. They are inoculated, incubated and assessed (see Table 4) in the same way as the test specimens in test series I. Should growth occur on these inert surfaces this indicates that nutrients have been unintentionally transported onto the surface of the control specimen with the inoculum. Any growth on these negative control specimens shall be reported according to Table 4 as well as whether growth is more or less than on the other inoculated specimens in test series I.
For visual examination, prepare a total of at least 11 specimens per plastic sample and per test method.Additionally, prepare 3 negative control specimens (stainless steel coupons), if testing according to Method A of this document. Prepare 5 specimens without biocide as positive controls, if testing for biocidal effects according to Method B. These coupons shall be cleaned according to EN 13697.
Dip specimens for methods A and C into the microbicidal solution (5.1.11) for 1 min and either dry at 45 °C for 4 h or, alternatively, for 72 hours at room temperature under sterile conditions, unless they are adversely affected by ethanol. In the latter case, store the specimens in a sterile container, handling them with sterile forceps. Carry out all subsequent handling of the specimens using forceps to avoid contamination by extraneous organic matter.ISO 846 pdf free download.