BS ISO 20752:2014 pdf free
BS ISO 20752:2014 pdf free.Cork stoppers一Determination of releasable 2, 4, 6-trichloroanisol (TCA)
The set of calibration solutions for TCA is obtained by adding known concentrations of analyte to the wine simulant. Standard solutions from 0,5 ng/L to 10 ng/L can be used.
The calibration curve obtained shall be evaluated regularly and whenever any large change is recorded by the GC-MS or GC/ECD.
Intact straight cork stoppers are subjected to maceration for 24 h±2 h at room temperature, using enough wine simulant, to keep the stoppers entirely immersed, and then analyse the macerate.
EXAMPLE 1 20 corks (45×24) mm, in a 1 L flask.
EXAMPLE 2 50 stoppers (45×24) mm, in a 2 L flask.
For bar-top stoppers, cut off the flange before maceration.
For granulated cork, 40 g of granulated cork are subjected to maceration for 24 h±2 h at room temperature in a 2 L flask, using enough wine simulant to keep the granulated cork entirely immersed, and then analyse the macerate.
For cork discs, 20 cork discs are subjected to maceration for 24 h±2 h at room temperature ina 0,5 L flask, using enough wine simulant to keep the granulated cork entirely immersed, and then analyse the macerate.
For cork stoppers for sparkling wines, immerse in the wine simulant only the discs of natural cork and 1 cm of the agglomerate without cutting the stoppers, for 24 h±2 h at room temperature, and then analyse the macerate.
Take a volume of the solution to be tested not greater than 50 % of the vial capacity (7.3).
EXAMPLE 1 For a vial [7.3) of 20 mL, take 10 mL, in such a way that the fibre does not touch the liquid.
The test portion should be saturated with NaCl (approximately 0,30 g/mL).
Add the internal standard solution, 12 %, v/v ethanol/water, to the test portion in the vial (7.3) in such a way that the ethanol content and the total volume of the liquid do not change significantly.
EXAMPLE2 100 μL internal standard solution/10 mL total liquid.
The concentration of the internal standard solution shall be in the calibration curve range and shall be as close as possible to the specification.BS ISO 20752 pdf download.
