ISO 20536:2017 pdf free

ISO 20536:2017 pdf free.Footwear一Critical substances potentially present in footwear and footwear components
The sample is cut in small pieces and extracted with a mixture of toluene/acetone (80/20) percent by volume, in a sealed vial at 60 °C in an ultrasonic bath for 1 h.
The extract is analysed by gas chromatography/mass spectrometry (GC-MS).
The method uses an extraction surrogate (Phenol-D6), added just before the extraction step and quantified similar to a target compound. It is required to check the extraction efficiency and to calculate a recovery rate.
Anthracene-D10 is used as the internal standard and is added to the vial (sample and calibration) just before the chromatographic analysis. It is also used to make the calibration curve.Weigh 10 mg of Anthracene-D10 in a 10 ml volumetric flask and fill to the mark with acetone. Transfer the content to an amber 10 ml vial with a polytetrafluoroethylene (PTFE) stopper and store at /FREESPACE2ULSTANDARDSPDF/ISO/ISO-20536-2017.rar4 °C.
Prepare a 1:100 dilution of the stock solution (5.2.1). For example, take 100 ul of the stock solution in a 10 ml volumetric flask and fill to the mark with acetone.
Weigh 10 mg of Phenol-D6 in a 10 ml volumetric flask and fill to the mark with acetone. Transfer the content to an amber 10 ml vial with PTFE stopper and store at4 °C.
Prepare a 1:100 dilution of the stock solution (5.2.3). For example, take 100 μl of the stock solution in a 10 ml volumetric flask and fill to the mark with acetone.
Weigh 10 mg of phenol in a 10 ml volumetric flask and fill to the mark with acetone. Transfer the content to an amber 10 ml vial with PTFE stopper and store at 4 °C.
Weigh 1 g of the sample in a 20 ml glass vial (6.6). Then add 9,8 ml of the extraction mixture (5.1.6) and 200 μl of the extraction surrogate working solution (5.2.4).
The extraction is conducted with an ultrasonic device at 60 °C±5 °C during 60 min±2 min.
NOTE It has been proven that acetone is efficient as extraction solvent. However, its high polarity (compared to the extraction mixture) can conduct to a high amount of co-extracted compounds (such as dyes or other polar compounds like glycols) which could disturb the analysis.Some polymeric materials can be dissolved in the extraction mixture and can cause problems during the quantification. In these cases, precipitation by methanol could be necessary. Precipitate the solution obtained by adding 20 ml of methanol (5.1.7) and allow the precipitate to settle for at least 5 min.Filtrate, if necessary, using a 0,45 μm PTFE filter.ISO 20536 pdf free.